DNA dynamics and the measurement problem in protein force spectroscopy

Roland Netz, TU Munich

The dynamics of DNA involves thermal, elastic and hydrodynamic effects on different length and time scales, theoretical approaches thus rely on a combination of simulations, theory and scaling: - The local dynamics of DNA is scale dependent and exhibits elastic effects, hydrodynamic interactions and center-of-mass dynamics as one goes from smaller to larger scales. A dynamic mean-field approach is validated by hydrodynamic simulations and quantitatively compare with recent fluorescence-correlation spectroscopy data. Problems in experimental data are discerned. - In modern single-molecule force-spectroscopic studies of RNA or protein unfolding DNA functions as force-transducer. Extracting folding landscapes and transition rates requires a novel dynamic deconvolution approach. The dynamic response functions of DNA needed for this are supplied by theory.